Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model

dc.creatorStringa, Pablo Luis
dc.creatorRomanin, David Emmanuel
dc.creatorLausada, Natalia Raquel
dc.creatorPapa Gobbi, Rodrigo
dc.creatorZanuzzi, Carolina Natalia
dc.creatorMartín, Pedro
dc.creatorAbate Zárate, Juan Cruz
dc.creatorCabanne, Ana
dc.creatorArnal, Nathalie
dc.creatorVecchio Dezillio, Leandro Emmanuel
dc.creatorMilesi, María Verónica
dc.creatorPortiansky, Enrique Leo
dc.creatorGondolesi, Gabriel
dc.creatorRumbo, Martín
dc.date2017
dc.date2019-08-20T16:59:49Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/79408
dc.descriptionBackground. Intestinal transplantation (ITx) faces many challenges due to the complexity of surgery and to the multiple immunological reactions that lead to the necessity of rigorous follow-up for early detection of acute cellular rejection (ACR). Our aim was to determine the kinetics of ACR using an experimental ITx model, with emphasis in the characterization of the process using different approaches, including the use of functional assays of absorptive and barrier function.Methods. ITx in rats conducting serial sampling was performed. Clinical monitoring, graft histology, proinflammatory gene expression, and nitrosative stress determination were performed. Also, glucose absorption, barrier function using ovalbumin translocation, and contractile function were analyzed. Results. The model used reproduced the different stages of ACR. Allogeneic ITx recipients showed signs of rejection from postoperative day (POD) 5, with increasing severity until 12 POD. Histological evaluation showed mild rejection in early sampling and severe rejection at late stages, with alterations in all graft layers. IL-6, CXCL 10, IFNg, and nitrite plasmas levels showed behavior coincident with histopathology. Remarkably, allogeneic grafts showed a marked alteration of glucose absorptive capacity from POD 5 that was sustained until endpoint. Coincidently, barrier function alteration was evidenced by luminal ovalbumin translocation to serum. Contractile function was progressively impaired along ACR. Conclusions. Glucose absorption and barrier function are altered at early stages of ACR when histological alterations or gene expression changes were much subtle. This observation may provide simple evaluation tools that could be eventually translated to the clinics to contribute to early ACR diagnosis.
dc.descriptionFacultad de Ciencias Médicas
dc.descriptionInstituto de Estudios Inmunológicos y Fisiopatológicos
dc.descriptionLaboratorio y Programa de Trasplante de Organos, Tejidos y Células
dc.descriptionInstituto de Investigaciones Bioquímicas de La Plata
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)
dc.subjectCiencias Médicas
dc.subjectIntestinal transplantation
dc.subjectacute cellular rejection
dc.titleGut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
dc.typeArticulo
dc.typeArticulo


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