dc.creator | Cortizo, Ana María | |
dc.creator | Caporossi, Mariana | |
dc.creator | Lettieri, Gabriela | |
dc.creator | Etcheverry, Susana B. | |
dc.date | 2000 | |
dc.date | 2019-06-11T19:26:03Z | |
dc.identifier | http://sedici.unlp.edu.ar/handle/10915/76265 | |
dc.identifier | issn:0014-2999 | |
dc.description | Nitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO production in two osteoblast-like cells UMR106 and MC3T3E1. incubated with different concentrations 2.5–100 mM. of vanadate. Vanadate induced NO release in a biphasic manner, with levels being significantly increased at concentrations over 50 mM. The NO donor, sodium nitroprusside, mimicked the vanadate effect: it inhibited cell growth and alkaline phosphatase activity in a dose-dependent manner. Vanadate enhanced the NO synthases, the endothelial and inducible eNOS and iNOS. isoforms, in a dose-dependent manner. Experiments performed with the ionophore A23187 and EGTA suggested that vanadate-induced NO production involves Ca2q-dependent and -independent mechanisms. Altogether, our results suggest that NO may play a critical role in the bioactivity of vanadium in osteoblast-like cells. | |
dc.description | Facultad de Ciencias Exactas | |
dc.format | application/pdf | |
dc.format | 279-285 | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
dc.subject | Ciencias Exactas | |
dc.subject | Nitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity | |
dc.subject | Química | |
dc.title | Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation | |
dc.type | Articulo | |
dc.type | Articulo | |