Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation

dc.creatorCortizo, Ana María
dc.creatorCaporossi, Mariana
dc.creatorLettieri, Gabriela
dc.creatorEtcheverry, Susana B.
dc.date2000
dc.date2019-06-11T19:26:03Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/76265
dc.identifierissn:0014-2999
dc.descriptionNitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO production in two osteoblast-like cells UMR106 and MC3T3E1. incubated with different concentrations 2.5–100 mM. of vanadate. Vanadate induced NO release in a biphasic manner, with levels being significantly increased at concentrations over 50 mM. The NO donor, sodium nitroprusside, mimicked the vanadate effect: it inhibited cell growth and alkaline phosphatase activity in a dose-dependent manner. Vanadate enhanced the NO synthases, the endothelial and inducible eNOS and iNOS. isoforms, in a dose-dependent manner. Experiments performed with the ionophore A23187 and EGTA suggested that vanadate-induced NO production involves Ca2q-dependent and -independent mechanisms. Altogether, our results suggest that NO may play a critical role in the bioactivity of vanadium in osteoblast-like cells.
dc.descriptionFacultad de Ciencias Exactas
dc.formatapplication/pdf
dc.format279-285
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectCiencias Exactas
dc.subjectNitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity
dc.subjectQuímica
dc.titleVanadate-induced nitric oxide production: role in osteoblast growth and differentiation
dc.typeArticulo
dc.typeArticulo


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