Artículos de revistas
Soil metagenomics reveals differences under conventional and no-tillage with crop rotation or succession.
Registro en:
34508
10.1016/j.apsoil.2013.05.021
Autor
SOUZA, R. C.
CANTAO, M. E.
VASCONCELOS, A. T. R.
NOGUEIRA, M. A.
HUNGRIA, M.
Institución
Resumen
Soil conservation practices are critical for agricultural sustainability, and in this study the shotgunsequencing approach was used to investigate the effects on soil biodiversity of different soil- andcrop-management practices in a 13-year field trial in southern Brazil. Treatments consisted of conven-tional tillage (CT) with plowing and disking, or no-tillage (NT) with direct sowing into the residues ofprevious crops, in a crop succession [soybean (summer)/wheat (winter)] or rotation [soybean/maize(summer)/wheat/lupine/oat (winter)]. About 1 million reads per treatment revealed very high levels ofdiversity. The majority of the sequences were attributed to the Bacteria (54%), and 0.3% and 0.2% fittedinto Archaea and Eucarya domains, respectively; 46% showed no similarity with any known sequences.Major differences were associated with tillage and, to a lesser extent, with crop management. Statisticallysignificant higher abundances with CT encompassed microorganisms associated with residue decompo-sition, carbon and nitrogen cycling, and xenobiosis. Eucarya were also more abundant with CT, possiblyrelated to higher tolerance of environmental stresses. In contrast, NT showed higher abundances par-ticularly of nitrogen-fixing Rhizobiales and Archaea that inhabit environments rich in organic matter. 2013