dc.description.abstract | Essential oils from pipilongo seeds (Piper tuberculatum) was extracted using supercritical
carbon dioxide. The extraction was performed as a function of particle size of the grinded seeds.
The highest yield (2,812%) was obtained with the smallest particle size. The chemical composition
analysis of the oil by GC-MS led to identify 15 compounds, some of which are β-elemene,
caryophyllene, β-farnesene, neophytadiene and piperine among others. The microbicide activity
of the essential oil was determined by Minimum Inhibitory Concentration (MIC) and Minimum
Bactericidal Concentration (MBC) assays, showing that the growth of the bacteria Staphylococcus
aureus and Bacillus subtilis was inhibited, and hence with a possible microbicidal effect, whereas
for pseudomonas aeruginosa and Salmonella typhimurium showed no effect on their growth. | |