Artigo
Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury
Fecha
2014-01-01Registro en:
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 60-67, 2014.
0102-8650
S0102-86502014001500060.pdf
S0102-86502014001500060
10.1590/S0102-86502014001700012
WOS:000344466300013
Autor
Corrêa, Silvana Aparecida Alves [UNIFESP]
Noronha, Samuel Marcos Ribeiro de
Lanziani, Larissa Elias
Ferreira, Lydia Masako [UNIFESP]
Gragnani, Alfredo [UNIFESP]
Institución
Resumen
PURPOSE:Evaluate the expression profile of genes related to Innate and Adaptive Immune System (IAIS) of human Primary Epidermal keratinocytes (hPEKP) of patients with severe burns.METHODS: After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(r) (Life Technologies) for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific IAIS PCR Arrays plates (SA Biosciences).RESULTS: After the analysis of gene expression we found that 63% of these genes were differentially expressed, of which 77% were repressed and 23% were hyper-regulated. Among these, the following genes (fold increase or decrease): IL8 (41), IL6 (32), TNF (-92), HLA-E (-86), LYS (-74), CCR6 (- 73), CD86 (-41) and HLA-A (-35).CONCLUSIONS: This study contributes to the understanding of the molecular mechanisms underlying wound infection caused by the burn. Furthermore, it may provide new strategies to restore normal expression of these genes and thereby change the healing process and improve clinical outcome.