dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorBorges, Fernanda Teixeira
dc.creatorDalboni, Maria Aparecida
dc.creatorMichelacci, Yara Maria
dc.creatorSchor, Nestor
dc.date.accessioned2015-06-14T13:41:54Z
dc.date.accessioned2019-05-24T16:56:59Z
dc.date.available2015-06-14T13:41:54Z
dc.date.available2019-05-24T16:56:59Z
dc.date.created2015-06-14T13:41:54Z
dc.date.issued2010-10-01
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 43, n. 10, p. 957-963, 2010.
dc.identifier0100-879X
dc.identifierhttp://repositorio.unifesp.br/handle/11600/5951
dc.identifierS0100-879X2010001000006.pdf
dc.identifierS0100-879X2010001000006
dc.identifier10.1590/S0100-879X2010007500095
dc.identifierWOS:000283263500006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2825203
dc.description.abstractHyperuricemia is associated with renal stones, not only consisting of uric acid (UrAc) but also of calcium oxalate (CaOx). Glycosaminoglycans (GAGs) are well-known inhibitors of growth and aggregation of CaOx crystals. We analyzed the effect of noncrystalline UrAc on GAG synthesis in tubular distal cells. MDCK (Madin-Darby canine kidney) cells were exposed to noncrystalline UrAc (80 µg/mL) for 24 h. GAGs were labeled metabolically and characterized by agarose gel electrophoresis. The expression of proteoglycans and cyclooxygenase 2 (COX-2) was assessed by real-time PCR. Necrosis, apoptosis and prostaglandin E2 (PGE2) were determined by acridine orange, HOESCHT 33346, and ELISA, respectively. CaOx crystal endocytosis was evaluated by flow cytometry. Noncrystalline UrAc significantly decreased the synthesis and secretion of heparan sulfate into the culture medium (UrAc: 2127 ± 377; control: 4447 ± 730 cpm) and decreased the expression of perlecan core protein (UrAc: 0.61 ± 0.13; control: 1.07 ± 0.16 arbitrary units), but not versican. Noncrystalline UrAc did not induce necrosis or apoptosis, but significantly increased COX-2 and PGE2 production. The effects of noncrystalline UrAc on GAG synthesis could not be attributed to inflammatory actions because lipopolysaccharide, as the positive control, did not have the same effect. CaOx was significantly endocytosed by MDCK cells, but this endocytosis was inhibited by exposure to noncrystalline UrAc (control: 674.6 ± 4.6, CaOx: 724.2 ± 4.2, and UrAc + CaOx: 688.6 ± 5.4 geometric mean), perhaps allowing interaction with CaOx crystals. Our results indicate that UrAc decreases GAG synthesis in MDCK cells and this effect could be related to the formation of UrAc and CaOx stones.
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectNoncrystalline uric acid
dc.subjectGlycosaminoglycan
dc.subjectMDCK
dc.subjectLipopolysaccharide
dc.subjectApoptosis
dc.subjectProteoglycan
dc.titleNoncrystalline uric acid inhibits proteoglycan and glycosaminoglycan synthesis in distal tubular epithelial cells (MDCK)
dc.typeArtículos de revistas


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