TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorInstituto Ludwig de Pesquisa sobre o Câncer
dc.creatorDalla Torre, Cristiane Arruda [UNIFESP]
dc.creatorMaciel, Rui Monteiro de Barros [UNIFESP]
dc.creatorPinheiro, Nídia Alice [UNIFESP]
dc.creatorAndrade, Joyce Anderson Duffles [UNIFESP]
dc.creatorToledo, Silvia Regina Caminada de [UNIFESP]
dc.creatorVilla, Luisa Lina
dc.creatorCerutti, Janete Maria [UNIFESP]
dc.date.accessioned2015-06-14T13:29:34Z
dc.date.available2015-06-14T13:29:34Z
dc.date.created2015-06-14T13:29:34Z
dc.date.issued2002-01-01
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 65-68, 2002.
dc.identifier0100-879X
dc.identifierhttp://repositorio.unifesp.br/handle/11600/1315
dc.identifierS0100-879X2002000100009.pdf
dc.identifierS0100-879X2002000100009
dc.identifier10.1590/S0100-879X2002000100009
dc.identifierWOS:000173942600009
dc.description.abstractMeasurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required.
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectTelomeric repeat amplification protocol
dc.subjectTRAP assay
dc.subjectHuman thyroid cancer
dc.subjectLeukemia
dc.subjectTelomerase
dc.subjectSilver staining
dc.titleTRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
dc.typeArtigo


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