| dc.creator | Teijeiro, Juan Manuel | |
| dc.creator | Marini, Patricia Estela | |
| dc.creator | Bragado, María Julia | |
| dc.creator | García-Marin, Luis | |
| dc.date | 2017-11-17T01:26:06Z | |
| dc.date | 2017-11-17T01:26:06Z | |
| dc.date | 2017-02-10 | |
| dc.date | 2017-11-17T01:26:06Z | |
| dc.date | 2017-11-17T01:26:06Z | |
| dc.date | 2017-02-10 | |
| dc.date.accessioned | 2019-05-17T20:22:05Z | |
| dc.date.available | 2019-05-17T20:22:05Z | |
| dc.identifier | 2047-2927 | |
| dc.identifier | http://hdl.handle.net/2133/9294 | |
| dc.identifier | http://hdl.handle.net/2133/9294 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2679599 | |
| dc.description | Male germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes
are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key
regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a
role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate
13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm.
In order to gain knowledge about those cellular processes regulated by PKC, we evaluate the effects of PMA on boar sperm motility,
lipid organization of plasma membrane, integrity of acrosome membrane and sperm agglutination. Also, we investigate the crosstalk
between PKA and PKC intracellular pathways in spermatozoa from this species. The results presented here reveal a participation of
PKC in sperm motility regulation and membrane fluidity changes, which is probably associated to acrosome reaction and to agglutination.
Also, we show the existence of a hierarchy in the kinases pathway. Previous works on boar sperm suggest a pathway in which
PKA is positioned upstream to PKC and this new results support such model. | |
| dc.description | Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva (CONICET); Argentina | |
| dc.description | Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva (CONICET); Argentina | |
| dc.description | Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina | |
| dc.description | Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad Nacional de Rosario; Argentina | |
| dc.description | Fil: Bragado, María Julia. Universidad de Extremadura. Escuela de Medicina Veterinaria. Grupo de Investigación de Señalización Intracelular y Tecnología de la Reproducción; España | |
| dc.description | Fil: García-Marin, Luis. Universidad de Extremadura. Escuela de Medicina Veterinaria. Grupo de Investigación de Señalización Intracelular y Tecnología de la Reproducción; España | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Wiley | |
| dc.relation | http://onlinelibrary.wiley.com/doi/10.1111/andr.12312/abstract | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
| dc.rights | Wiley | |
| dc.rights | https://authorservices.wiley.com/asset/photos/licensing-and-open-access-photos/eCTA_sample.pdf | |
| dc.rights | embargoedAccess | |
| dc.subject | Espermatozoides porcinos | |
| dc.subject | Fluidez de membrana | |
| dc.subject | Protein quinasa C | |
| dc.subject | Boar sperm | |
| dc.subject | Membrane fluidity | |
| dc.subject | Protein kinase C | |
| dc.title | Protein kinase C activity in boar sperm | |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
