Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines.

dc.contributorEmbrapa Milho e Sorgo
dc.creatorPETRILLO, C.P.
dc.creatorCARNEIRO, N.P.
dc.creatorPURCINO, A.A.C.
dc.creatorCARVALHO, C.H.S.
dc.creatorALVES, J.D.
dc.creatorCARNEIRO, A.A.
dc.date2008
dc.date2008-06-09
dc.date.accessioned2017-03-06T19:27:23Z
dc.date.available2017-03-06T19:27:23Z
dc.identifier43238
dc.identifierhttp://www.alice.cnptia.embrapa.br/handle/doc/124191
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/266009
dc.descriptionThe objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.
dc.description2008
dc.languageen
dc.publisherPesquisa Agropecuária Brasileira, Brasilia, DF, v. 43, n.3, p.371-378, mar. 2008.
dc.relationÁrea de Informação da Sede - Artigo em periódico indexado (ALICE)
dc.subjectZea mays
dc.subjectammonium glufosinate
dc.subjectbeta-glucuronidase
dc.subjectimmature embryos
dc.subjectphosphinotricin acetyl transferase
dc.subjectplant transformation
dc.subjectglufosinato de amônio
dc.subjectbeta-glucuronidase
dc.subjectembriões imaturos
dc.subjectfosfinotricina acetil transferase
dc.subjecttransformação de plantas
dc.titleOptimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines.
dc.typeArtículos de revistas


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