Artículos de revistas
Development of HA/Ag-NPs composite coating from green process for hip applications
Fecha
2017-08-01Registro en:
Molecules, v. 22, n. 8, 2017.
1420-3049
10.3390/molecules22081291
2-s2.0-85028358138
2-s2.0-85028358138.pdf
Autor
Centro de Investigación en Materiales Avanzados (CIMAV)
Universidade de Sorocaba (UNISO)
Universidade Estadual Paulista (Unesp)
Centro de Investigación en Quîmica Aplicada (CIQA)
Universidad Tecnológica de Chihuahua Sur (UTCh Sur)
Institución
Resumen
In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 Antimicrobial Product-Test for Antimicrobial Activity and Efficacy. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry.