dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:33:25Z
dc.date.available2018-12-11T17:33:25Z
dc.date.created2018-12-11T17:33:25Z
dc.date.issued2017-09-01
dc.identifierMicrobial Pathogenesis, v. 110, p. 507-511.
dc.identifier1096-1208
dc.identifier0882-4010
dc.identifierhttp://hdl.handle.net/11449/179067
dc.identifier10.1016/j.micpath.2017.07.045
dc.identifier2-s2.0-85026554273
dc.identifier2-s2.0-85026554273.pdf
dc.description.abstractPorphyromonas gingivalis is an important pathogen in the development of periodontal disease. Our study investigated if the treatment with antimicrobial photodynamic therapy (aPDT) that employs a nontoxic dye, followed by irradiation with harmless visible light can attenuate the experimental infection of P. gingivalis in Galleria mellonella. Firstly, different concentrations of P. gingivalis ranging from 102 to 106 cells/larva were injected into the animal to obtain a lethal concentration. Next, the following groups of G. mellonella infected with P. gingivalis were evaluated: inoculation of the photosensitizer and application of laser (P + L+), inoculation of physiologic solution and application of laser (P-L+), inoculation the photosensitizer without laser (P + L-) and inoculation of physiologic solution without Laser (P-L-). The effects of aPDT on infection by P. gingivalis were evaluated by survival curve analysis and hemocytes count. A lethal concentration of 106 cells/larva was adopted for evaluating the effects of aPDT on experimental infection with P. gingivalis. We found that after 120 s of PDT application, the death of G. mellonella was significantly lower compared to the control groups (p = 0.0010). Moreover, the hemocyte density in the P+L+ group was increased by 9.6 × 106 cells/mL (2.62-fold increase) compared to the infected larvae with no treatment (L-P- group) (p = 0.0175). Finally, we verified that the aPDT led to a significant reduction of the number of P. gingivalis cells in G. mellonella hemolymph. In conclusion, PDT application was effective against P. gingivalis infection by increasing the survival of G. mellonella and was able to increase the circulating hemocytes indicating that PDT activates the G. mellonella immune system.
dc.languageeng
dc.relationMicrobial Pathogenesis
dc.relation0,751
dc.relation0,751
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectGalleria mellonella
dc.subjectPhotodynamic therapy
dc.subjectPorphyromonas gingivalis
dc.titleImmunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución