dc.contributor | Universidade Federal de Pernambuco (UFPE) | |
dc.contributor | Polytechnic Institute of Porto | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2018-12-11T17:26:01Z | |
dc.date.available | 2018-12-11T17:26:01Z | |
dc.date.created | 2018-12-11T17:26:01Z | |
dc.date.issued | 2016-03-15 | |
dc.identifier | Biosensors and Bioelectronics, v. 77, p. 978-985. | |
dc.identifier | 1873-4235 | |
dc.identifier | 0956-5663 | |
dc.identifier | http://hdl.handle.net/11449/177571 | |
dc.identifier | 10.1016/j.bios.2015.10.068 | |
dc.identifier | 2-s2.0-84946017754 | |
dc.identifier | 2-s2.0-84946017754.pdf | |
dc.description.abstract | A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1ngmL-1 (r=0.995, p«0.01), with a very low limit of detection (0.006ngmL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (KD=7.3 10-13molL-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications. | |
dc.language | eng | |
dc.relation | Biosensors and Bioelectronics | |
dc.relation | 2,373 | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Acid carboxylic-3-pyrrole | |
dc.subject | Cardiac troponin T | |
dc.subject | Graphene | |
dc.subject | Molecularly imprinted polymer | |
dc.subject | Point-of-care testing | |
dc.subject | Pyrrole | |
dc.title | An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer | |
dc.type | Artículos de revistas | |