Artículos de revistas
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma
Fecha
2016-11-01Registro en:
Tumor Biology, v. 37, n. 11, p. 15087-15096, 2016.
1423-0380
1010-4283
10.1007/s13277-016-5356-8
2-s2.0-84988723057
Autor
University of S�o Paulo
Stem Cell and Cell Therapy and Center for Cell Based Therapy
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Memorial Sloan-Kettering Cancer Center
University of Centro-Oeste
Centro Regional de Hemoterapia de Ribeir�o Preto
Institución
Resumen
Laryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be a subtype of the head and neck squamous cell carcinoma (HNSCC). Despite significant advances in the understanding and treatment of cancer, prognosis of patients with LSCC has not improved recently. In the present study, we sought to understand better the genetic mechanisms underlying LSCC development. Thirty-two tumor samples were collected from patients undergoing surgical resection of LSCC. The samples were submitted to whole-genome cDNA microarray analysis aiming to identify genetic targets in LSCC. We also employed bioinformatic approaches to expand our findings using the TCGA database and further performed functional assays, using human HNSCC cell lines, to evaluate viability, cell proliferation, and cell migration after silencing of selected genes. Eight members of the homeobox gene family (HOX) were identified to be overexpressed in LSCC samples when compared to normal larynx tissue. Quantitative RT-PCR analysis validated the overexpression of HOX gene family members in LSCC. Receiver operating characteristic (ROC) statistical method curve showed that the expression level of seven members of HOX gene family can distinguish tumor from nontumor tissue. Correlation analysis of clinical and gene expression data revealed that HOXC8 and HOXD11 genes were associated with the differentiation degree of tumors and regional lymph node metastases, respectively. Additionally, siRNA assays confirmed that HOXC8, HOXD10, and HOXD11 genes might be critical for cell colony proliferation and cell migration. According to our findings, several members of the HOX genes were overexpressed in LSCC samples and seem to be required in biological processes involved in tumor development. This suggests that HOX genes might play a critical role in the physiopathology of LSCC tumors.