Artículos de revistas
Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold
Fecha
2017-09-01Registro en:
Acta Cirurgica Brasileira, v. 32, n. 9, p. 706-711, 2017.
1678-2674
0102-8650
10.1590/s0102-865020170090000003
S0102-86502017000900706
2-s2.0-85031122144
S0102-86502017000900706.pdf
1109525021631011
9609324832591382
4513014379461383
0000-0003-3775-3797
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1-control, consisting of samples of vena cava in natura; group 2-SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm -Shaker News Brunswick Scientific*) for 1 hour at controlled temperature shaker at 37°C; group 3-SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.