dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Melchor Fernández Almagro | |
dc.date.accessioned | 2018-12-11T17:03:01Z | |
dc.date.available | 2018-12-11T17:03:01Z | |
dc.date.created | 2018-12-11T17:03:01Z | |
dc.date.issued | 2016-01-01 | |
dc.identifier | Materials, v. 9, n. 5, 2016. | |
dc.identifier | 1996-1944 | |
dc.identifier | http://hdl.handle.net/11449/172990 | |
dc.identifier | 10.3390/ma9050357 | |
dc.identifier | 2-s2.0-84969909301 | |
dc.identifier | 2-s2.0-84969909301.pdf | |
dc.description.abstract | In this study, trypsin (Enzyme Comission 3.4.21.4) was immobilized in a low cost, lignocellulosic support (corn cob powder-CCP) with the goal of obtaining peptides with bioactive potential from cheese whey. The pretreated support was activated with glyoxyl groups, glutaraldehyde and IDA-glyoxyl. The immobilization yields of the derivatives were higher than 83%, and the retention of catalytic activity was higher than 74%. The trypsin-glyoxyl-CCP derivative was thermally stable at 65 °C, a value that was 1090-fold higher than that obtained with the free enzyme. The trypsin-IDA-glyoxyl-CCP and trypsin-glutaraldehyde-CCP derivatives had thermal stabilities that were 883-and five-fold higher, respectively, then those obtained with the free enzyme. In the batch experiments, trypsin-IDA-glyoxyl-CCP retained 91% of its activity and had a degree of hydrolysis of 12.49%, while the values for trypsin-glyoxyl-CCP were 87% and 15.46%, respectively. The stabilized derivative trypsin-glyoxyl-CCP was also tested in an upflow packed-bed reactor. The hydrodynamic characterization of this reactor was a plug flow pattern, and the kinetics of this system provided a relative activity of 3.04 ± 0.01 U· g-1 and an average degree of hydrolysis of 23%, which were suitable for the production of potentially bioactive peptides. | |
dc.language | eng | |
dc.relation | Materials | |
dc.relation | 0,732 | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Corn cob powder functionalized | |
dc.subject | Immobilization | |
dc.subject | Peptides | |
dc.subject | Reactor | |
dc.subject | Trypsin | |
dc.subject | Whey protein hydrolysates | |
dc.title | Immobilization of trypsin in lignocellulosic waste material to produce peptides with bioactive potential from whey protein | |
dc.type | Artículos de revistas | |