Artículos de revistas
Treatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccine
Fecha
2017-07-01Registro en:
Oncology Reports. Athens: Spandidos Publ Ltd, v. 38, n. 1, p. 561-568, 2017.
1021-335X
10.3892/or.2017.5692
WOS:000404089500064
8845835550637809
0000-0002-4292-3298
Autor
Universidade Estadual Paulista (Unesp)
Univ Florence
Careggi Univ Hosp AOUC
Max Planck Inst Immunobiol & Epigenet
Institución
Resumen
Non-cytotoxic concentrations of selected chemotherapeutic agents amplify the antigen presentation capacity of dendritic cells (DCs) and are able to increase the immunogenicity of the colon cancer cell lineage HCT-116, as previously demonstrated by our group. Since this functional alteration was associated with changes in gene expression, we aimed to evaluate whether transcriptional changes of tumor cells can be transferred to DCs, increasing their ability to induce a specific antitumor response. Therefore, HCT-116 cells were treated with two different concentrations of 5-fluorouracil (5-FU), and their total RNA was transfected into human monocyte-derived DC, which function was evaluated through their ability to stimulate the proliferation of normal allogeneic T lymphocytes (MLR), and to generate cytolytic T cells. The transfected DCs demonstrated an increased percentage of CD83(+), HLA-DR+, CD80(+) and CD86(+) cells. These phenotypical changes were followed by functional improvement demonstrated by the increased capacity of these DC to induce allogeneic T cell proliferation and to generate specific anti-HCT-116 cytolytic T cells, as demonstrated by IFN-gamma production following in vitro challenge with tumor cells. Our results allow us to conclude that treatment of tumor cells with a non-toxic concentration of 5-FU induces immunogenic changes that are transferred to DC by transfection of total RNA.