dc.contributorUniv Pernambuco UPE
dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:19:06Z
dc.date.available2018-11-26T16:19:06Z
dc.date.created2018-11-26T16:19:06Z
dc.date.issued2015-10-01
dc.identifierInternational Journal Of Experimental Pathology. Hoboken: Wiley-blackwell, v. 96, n. 5, p. 285-293, 2015.
dc.identifier0959-9673
dc.identifierhttp://hdl.handle.net/11449/161091
dc.identifier10.1111/iep.12142
dc.identifierWOS:000367324300002
dc.identifier3681613160086175
dc.identifier0000-0003-3944-1906
dc.description.abstractIn Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-alpha; pro-inflammatory cytokine), tumour growth factor (TGF-beta; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-alpha, TGF-beta and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-alpha and TGF-beta serve as markers of dystrophy primarily for the diaphragm.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationInternational Journal Of Experimental Pathology
dc.relation0,712
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcalsequestrin
dc.subjectdystrophy
dc.subjectmdx
dc.subjecttumour growth factor-beta
dc.subjecttumour necrosis factor-alpha
dc.titleChanges in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles
dc.typeArtículos de revistas


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