Artículos de revistas
DEVELOPMENT OF THE MAIN ORGANS IN BUFFALO EMBRYOS (Bubalus bubalis)
Buffalo Bulletin. Bangkok: Int Buffalo Information Ctr, v. 34, n. 4, p. 429-439, 2015.
Fed Univ Western Para UFOPA
Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
To give support for reproductive technology and to gain further information about buffalo morphology, we evaluated the morphological aspects of buffalo embryos and membranes of gestational age between 10-60 days. Ninety-six uteri at an early gestational stage from pregnant buffalos (Bubalus bubalis) were used. The pregnant horns were separated from the remainder of the uterus by means of clamps and were then perfused with 4% buffered paraformaldehyde or Bouin solution. Forty-eight hours after fixation by immersion, the pregnant uterine horn was incised, and the embryo with its fetal membranes was withdrawn. The embryos were distributed into groups according to their ages. Samples were also fixed in 10% formaldehyde and 2.5% glutaraldehyde and processed and stained, followed by examination by means of optical microscopy and immunohistochemistry. Despite some differences in the number of days, the development of the renal system in bovine and bubaline embryos is very similar, and these findings can be used to check the normality of embryo development by means of ultrasound and other imaging techniques. We identified the presence of pluripotent cells at different gestational ages and in different regions of the buffalo embryos and fetal membranes. During the gestation, the number of positive cells decreases significantly but these cells do not disappear completely. Hemangioblast cells in young buffalos embryos can be considered to be pluripotent cells: some of these cells will migrate to the embryo through the primitive intestine and will remain in genital organs and in another few colonies distributed in the embryo.