Artículos de revistas
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
Fecha
2015-01-01Registro en:
Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015.
2314-6133
10.1155/2015/750752
WOS:000359237100001
WOS000359237100001.pdf
2514762545280942
0000-0002-1378-6327
Autor
Universidade de São Paulo (USP)
LIBBS Co
Universidade Estadual Paulista (Unesp)
Institución
Resumen
To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine.