dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorIn Vitro Brasil Ltda.
dc.date.accessioned2016-07-07T12:37:48Z
dc.date.available2016-07-07T12:37:48Z
dc.date.created2016-07-07T12:37:48Z
dc.date.issued2015
dc.identifierJournal of Stem Cells, v. 9, n. 4, p. 225-234, 2015.
dc.identifier1556-8539
dc.identifierhttp://hdl.handle.net/11449/141313
dc.identifier0711118889066020
dc.identifier1945811068525845
dc.identifier6130919869209146
dc.identifier5588332776413493
dc.identifier8456490300814833
dc.identifier0000-0002-2420-2550
dc.description.abstractThe objective of this study was to evaluate the potential of bovine IVF blastocysts at different stages of embryonic development in establishing ESC-like. Furthermore, blastocysts cultured in medium containing (10%) and (2.5%) fetal calf serum (FCS) were compared to determine if the serum concentration during in vitro culture alters the blastocyst's potential to establish ESC-like culture. It was observed that only ICM's from expanded blastocysts adhered to the monolayer (n=160) independent of the concentration of serum used during IVF culture. There were no observable differences in potential to establish ESC-like in embryos cultured with 2,5% or 10% FCS . The bFGF didn´t seems to be required for maintenance of bovine ESC-like regardless of culture conditions. Blastocysts and colonies in primary culture and after the first passage were positive for Oct4, Nanog, SSEA-3 and TRA-1-81. Expanded blastocysts gave rise to ESC-like colonies, and the addition of LIF was sufficient to maintain cells undifferentiated in culture.
dc.languageeng
dc.relationJournal of Stem Cells
dc.relation0,314
dc.rightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectEmbryonic stem cells
dc.subjectBovine
dc.subjectBlastocyst
dc.subjectFetal calf serum
dc.subjectLIF
dc.titleInfluence of culture medium and age of bovine blastocysts in established colonies of embryonic stem cells
dc.typeArtículos de revistas


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