dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | In Vitro Brasil Ltda. | |
dc.date.accessioned | 2016-07-07T12:37:48Z | |
dc.date.available | 2016-07-07T12:37:48Z | |
dc.date.created | 2016-07-07T12:37:48Z | |
dc.date.issued | 2015 | |
dc.identifier | Journal of Stem Cells, v. 9, n. 4, p. 225-234, 2015. | |
dc.identifier | 1556-8539 | |
dc.identifier | http://hdl.handle.net/11449/141313 | |
dc.identifier | 0711118889066020 | |
dc.identifier | 1945811068525845 | |
dc.identifier | 6130919869209146 | |
dc.identifier | 5588332776413493 | |
dc.identifier | 8456490300814833 | |
dc.identifier | 0000-0002-2420-2550 | |
dc.description.abstract | The objective of this study was to evaluate the potential of bovine IVF blastocysts at different stages of embryonic development in establishing ESC-like. Furthermore, blastocysts cultured in medium containing (10%) and (2.5%) fetal calf serum (FCS) were compared to determine if the serum concentration during in vitro culture alters the blastocyst's potential to establish ESC-like culture. It was observed that only ICM's from expanded blastocysts adhered to the monolayer (n=160) independent of the concentration of serum used during IVF culture. There were no observable differences in potential to establish ESC-like in embryos cultured with 2,5% or 10% FCS . The bFGF didn´t seems to be required for maintenance of bovine ESC-like regardless of culture conditions. Blastocysts and colonies in primary culture and after the first passage were positive for Oct4, Nanog, SSEA-3 and TRA-1-81. Expanded blastocysts gave rise to ESC-like colonies, and the addition of LIF was sufficient to maintain cells undifferentiated in culture. | |
dc.language | eng | |
dc.relation | Journal of Stem Cells | |
dc.relation | 0,314 | |
dc.rights | Acesso restrito | |
dc.source | Currículo Lattes | |
dc.subject | Embryonic stem cells | |
dc.subject | Bovine | |
dc.subject | Blastocyst | |
dc.subject | Fetal calf serum | |
dc.subject | LIF | |
dc.title | Influence of culture medium and age of bovine blastocysts in established colonies of embryonic stem cells | |
dc.type | Artículos de revistas | |