dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2016-01-28T16:53:42Z | |
dc.date.available | 2016-01-28T16:53:42Z | |
dc.date.created | 2016-01-28T16:53:42Z | |
dc.date.issued | 2011 | |
dc.identifier | Veterinary and Comparative Oncology, v. 9, n. 4, p. 251-259, 2011. | |
dc.identifier | 1476-5810 | |
dc.identifier | http://hdl.handle.net/11449/133114 | |
dc.identifier | 10.1111/j.1476-5829.2010.00260.x | |
dc.identifier | 4272904699684196 | |
dc.identifier | 2850453183724361 | |
dc.identifier | 6404176495306171 | |
dc.identifier | 4584674909952477 | |
dc.description.abstract | Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis. | |
dc.language | eng | |
dc.relation | Veterinary and Comparative Oncology | |
dc.relation | 2.270 | |
dc.rights | Acesso restrito | |
dc.source | Currículo Lattes | |
dc.title | Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour | |
dc.type | Artículos de revistas | |