Artículos de revistas
Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells
Fecha
2015-09-26Registro en:
Toxicon : Official Journal Of The International Society On Toxinology, v. 108, p. 126-133, 2015.
1879-3150
10.1016/j.toxicon.2015.09.031
8573195327542061
26410111
Autor
Universidade Federal do Ceará (UFC)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
Centro de Investigación Príncipe Felipe
Institución
Resumen
Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.