dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:37:32Z
dc.date.available2015-12-07T15:37:32Z
dc.date.created2015-12-07T15:37:32Z
dc.date.issued2015-09-03
dc.identifierSpectrochimica Acta. Part A, Molecular And Biomolecular Spectroscopy, v. 153, p. 386-392, 2015.
dc.identifier1873-3557
dc.identifierhttp://hdl.handle.net/11449/131556
dc.identifier10.1016/j.saa.2015.08.048
dc.identifier3192125098361121
dc.identifier5978908591853524
dc.identifier26344484
dc.identifier0000-0003-0197-7369
dc.description.abstractAn environmentally reliable analytical methodology was developed for direct quantification of tetracycline (TC) and oxytetracycline (OTC) using continuous flow injection analysis with spectrophotometric detection. The method is based on the diazo coupling reaction between the tetracyclines and diazotized sulfanilic acid in a basic medium, resulting in the formation of an intense orange azo compound that presents maximum absorption at 434nm. Experimental design was used to optimize the analytical conditions. The proposed technique was validated over the concentration range of 1 to 40μgmL(-1), and was successfully applied to samples of commercial veterinary pharmaceuticals. The detection (LOD) and quantification (LOQ) limits were 0.40 and 1.35μgmL(-1), respectively. The samples were also analyzed by an HPLC method, and the results showed agreement with the proposed technique. The new flow injection method can be immediately used for quality control purposes in the pharmaceutical industry, facilitating monitoring in real time during the production processes of tetracycline formulations for veterinary use.
dc.languageeng
dc.publisherElsevier B. V.
dc.relationSpectrochimica Acta. Part A, Molecular And Biomolecular Spectroscopy
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectFlow injection analysis (fia)
dc.subjectGreen chemistry
dc.subjectQuantification
dc.subjectSpectrophotometry
dc.subjectTetracyclines
dc.subjectVeterinary pharmaceuticals
dc.titleSimple and clean determination of tetracyclines by flow injection analysis
dc.typeArtículos de revistas


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