dc.contributor | Universidade Estadual do Ceará (UECE) | |
dc.contributor | Universidade Potiguar (UnP) | |
dc.contributor | Universidade Federal de Viçosa (UFV) | |
dc.contributor | Universidade Federal de Pernambuco (UFPE) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2015-10-21T13:11:12Z | |
dc.date.available | 2015-10-21T13:11:12Z | |
dc.date.created | 2015-10-21T13:11:12Z | |
dc.date.issued | 2015-01-01 | |
dc.identifier | Animal Reproduction Science. Amsterdam: Elsevier Science Bv, v. 152, p. 32-38, 2015. | |
dc.identifier | 0378-4320 | |
dc.identifier | http://hdl.handle.net/11449/128583 | |
dc.identifier | 10.1016/j.anireprosci.2014.11.010 | |
dc.identifier | WOS:000349054900005 | |
dc.description.abstract | A sequential medium with fibroblast growth factor-10 (FGF-10) and follicle stimulating hormone (FSH) was evaluated on the survival, ultrastructure, activation and growth rate of caprine preantral follicles submitted to long-term culture, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in alpha-MEM alone or supplemented with FGF-10 and/or FSH added sequentially on different days of culture. Ovarian fragments were cultured during the first (days 0-8) and second (days 8-16) halves of the culture period, generating 10 treatments: alpha-MEW+/alpha-MEM+ (cultured control), FSH/FSH, FSH/FGF-10, FSH/FSH + FGF-10, FGF-10/FGF-10, FGF-10/FSH, FGF-10/FSH + FGF-10, FSH + FGF-10/FSH + FGF-10, FSH + FGF-10/FSH and FSH + FGF-10/FGF-10. Follicle morphology, viability and ultrastructure were analyzed. The FSH/FGF-10 treatment showed a higher (P < 0.05) percentage of normal follicles compared to all other treatments. In addition, follicles from the FSH/FGF-10 treatment maintained ultrastructural integrity after the culture period. After 16 days of culture, the FSH/FGF-10 and FSH/FSH treatments showed a higher percentage of activation compared to the cultured control (alpha-MEW+/alpha-MEM+). Moreover, the FSH/FGF-10 treatment promoted greater follicular and oocyte diameters compared to the fresh control. In conclusion, this study showed that a sequential medium with FSH followed by FGF-10 (FSH/FGF-10 and FSH/FSH) maintains follicular viability and ultrastructure and promotes transition from the primordial to primary stage (activation) and growth in goat preantral follicles cultured in vitro. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Animal Reproduction Science | |
dc.relation | 1.647 | |
dc.relation | 0,704 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Caprine | |
dc.subject | Folliculogenesis | |
dc.subject | FSH | |
dc.subject | FGF-10 | |
dc.subject | Preantral follicle | |
dc.title | Effect of sequential medium with fibroblast growth factor-10 and follicle stimulating hormone on in vitro development of goat preantral follicles | |
dc.type | Artículos de revistas | |