dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:09:58Z
dc.date.available2015-10-21T13:09:58Z
dc.date.created2015-10-21T13:09:58Z
dc.date.issued2015-04-01
dc.identifierJournal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 35, n. 4, p. 277-282, 2015.
dc.identifier0737-0806
dc.identifierhttp://hdl.handle.net/11449/128452
dc.identifier10.1016/j.jevs.2015.01.014
dc.identifierWOS:000352690300004
dc.identifier0473846154288947
dc.identifier0640158639112242
dc.description.abstractThe penis and prepuce of the stallion have a high bacterial load on its surface, forming a natural microbial flora that contaminates the semen during ejaculation. Bacterial growth in semen may cause a decline on sperm quality, viability, and fertility and predisposes the occurrence of endometritis in inseminated mares. Thus, the aim of this study was to evaluate the effect of penile wash before semen collection, the addition of different commercial skim milk-based extenders containing antibiotics (BotuSemen and INRA96), and the removal of seminal plasma by filtration on the quality, viability, and bacterial proliferation on fresh and cooled stallion semen. Animals that were never submitted to penile wash before semen collection tended to have lower bacterial contamination in the ejaculate. Semen samples extended in BotuSemen showed superiority in total motility, progressive motility, average path velocity, and rapid sperm and lower bacterial contamination in relation to semen samples extended in INRA96 after 24 hours of cooling. No difference was found in these parameters between the storage temperatures (5 degrees C and 15 degrees C). Furthermore, the removal of seminal plasma by filtration reduced the bacterial load in semen after cooling. In conclusion, the penile wash before semen collection tended to reduce the bacterial growth in fresh semen. The use of a semen extender with appropriate antibiotics and removal of seminal plasma by filtration were effective in reducing the bacterial contamination and preserved the quality of cooled stallion semen. (C) 2015 Elsevier Inc. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal Of Equine Veterinary Science
dc.relation0.880
dc.relation0,390
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSemen
dc.subjectStallion
dc.subjectBacteria
dc.subjectFiltration
dc.subjectPenile wash
dc.titleControl Methods and Evaluation of Bacterial Growth on Fresh and Cooled Stallion Semen
dc.typeArtículos de revistas


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