Microbiological Assay for the Determination of Colistin Sulphate

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Metodista Piracicaba
dc.date.accessioned2014-12-03T13:11:44Z
dc.date.available2014-12-03T13:11:44Z
dc.date.created2014-12-03T13:11:44Z
dc.date.issued2014-01-01
dc.identifierCurrent Analytical Chemistry. Sharjah: Bentham Science Publ Ltd, v. 10, n. 3, p. 443-448, 2014.
dc.identifier1573-4110
dc.identifierhttp://hdl.handle.net/11449/113477
dc.identifierWOS:000337253600016
dc.description.abstractAn innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear ( r(2) = 0.9985), precise ( CV = 1.32%) and accurate ( 100.9 +/- 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate.
dc.languageeng
dc.publisherBentham Science Publ Ltd
dc.relationCurrent Analytical Chemistry
dc.relation1.000
dc.relation0,274
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAgar diffusion method
dc.subjectBordetella bronchiseptica
dc.subjectcolistin sulphate
dc.subjectmethod validation
dc.subjectmicrobiological assay
dc.subjectin vitro quantification
dc.titleMicrobiological Assay for the Determination of Colistin Sulphate
dc.typeArtículos de revistas


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