Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country

dc.contributorUniversidade Estadual de Maringá (UEM)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorState Univ Western Parana
dc.contributorUniversidade de São Paulo (USP)
dc.date.accessioned2014-12-03T13:11:42Z
dc.date.available2014-12-03T13:11:42Z
dc.date.created2014-12-03T13:11:42Z
dc.date.issued2013-01-01
dc.identifierBiomed Research International. New York: Hindawi Publishing Corporation, 6 p., 2013.
dc.identifier2314-6133
dc.identifierhttp://hdl.handle.net/11449/113446
dc.identifier10.1155/2013/391549
dc.identifierWOS:000326519700001
dc.identifierWOS000326519700001.pdf
dc.identifier2114570774349859
dc.description.abstractCurrent study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.relationBioMed Research International
dc.relation2.583
dc.relation0,935
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.titleEvaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country
dc.typeArtículos de revistas


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