dc.contributor | Univ Vale Paraiba | |
dc.contributor | Universidade Federal de Ouro Preto (UFOP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-12-03T13:10:59Z | |
dc.date.available | 2014-12-03T13:10:59Z | |
dc.date.created | 2014-12-03T13:10:59Z | |
dc.date.issued | 2014-01-01 | |
dc.identifier | Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 1, p. 50-59, 2014. | |
dc.identifier | 0100-879X | |
dc.identifier | http://hdl.handle.net/11449/112703 | |
dc.identifier | 10.1590/1414-431X20133028 | |
dc.identifier | S0100-879X2013005003028 | |
dc.identifier | WOS:000331907500007 | |
dc.identifier | S0100-879X2013005003028.pdf | |
dc.description.abstract | Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival. | |
dc.language | eng | |
dc.publisher | Associação Brasileira de Divulgação Científica (ABRADIC) | |
dc.relation | Brazilian Journal of Medical and Biological Research | |
dc.relation | 1.492 | |
dc.rights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Cellular and molecular analyses | |
dc.subject | Cell death | |
dc.subject | Cyclooxygenase-2 inhibitor | |
dc.subject | H9c2 cardiac cell line | |
dc.title | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms | |
dc.type | Artículos de revistas | |