dc.contributor | Diretoria Pesquisa & Desenvolvimento Fdn Ezequiel | |
dc.contributor | Universidade Federal de Minas Gerais (UFMG) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-12-03T13:10:33Z | |
dc.date.available | 2014-12-03T13:10:33Z | |
dc.date.created | 2014-12-03T13:10:33Z | |
dc.date.issued | 2014-02-01 | |
dc.identifier | Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 68, n. 1, p. 87-91, 2014. | |
dc.identifier | 0753-3322 | |
dc.identifier | http://hdl.handle.net/11449/112245 | |
dc.identifier | 10.1016/j.biopha.2013.12.004 | |
dc.identifier | WOS:000332448400014 | |
dc.identifier | 8334785337106990 | |
dc.description.abstract | Despite impressive research efforts, the biology of epithelial ovarian cancer (EOC) remains poorly understood and alterations in the expression of CASPASE-8 contribute to a worse tumor prognosis. This study assesses the methylation of the CpG island within the CASPASE-8 promoter and CASPASE-8 gene expression both in cystadenoma tumors and in primary and metastatic EOC. DNA and RNA were obtained from women with normal ovarian tissues (n = 18), ovarian serous cystadenoma tumors (n = 11) and EOC (n = 16) using Trizol (R). The methylation frequency of the CpG island in the CASPASE-8 promoter was assessed using the methylation-specific PCR assay after DNA bisulfite conversion. Quantitative PCR was performed to quantify the relative levels of CASPASE-8 in each sample. The differences between samples with each group were evaluated using the Mann-Whitney U and Kruskal-Wallis tests as indicated. Hemimethylation of the CASPASE-8 promoter was found in 11.8% of the normal ovary samples, 20% of the cystadenoma tumors and 20% of the metastatic EOC, while methylation of the CASPASE-8 promoter was absent in the EOC primary tissues (P = 0.047). An increased CASPASE-8 expression level was observed in all tumor groups. Significant differences were observed in the CASPASE-8 expression levels when compared with all ovarian tumor groups (P = 0.0278). Promoter DNA methylation did not associate with expression levels of CASPASE-8, suggesting the presence of other mechanisms in relation to gene expression control in EOC; thus providing a better understanding of this complex disease. (C) 2013 Elsevier Masson SAS. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Biomedicine & Pharmacotherapy | |
dc.relation | 3.457 | |
dc.relation | 0,951 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Epithelial ovarian cancer | |
dc.subject | CASPASE-8 | |
dc.subject | Expression | |
dc.title | Single CpG island methylation is not sufficient to maintain the silenced expression of CASPASE-8 apoptosis-related gene among women with epithelial ovarian cancer | |
dc.type | Artículos de revistas | |