dc.contributor | Universidade Federal de São Carlos (UFSCar) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-12-03T13:08:54Z | |
dc.date.available | 2014-12-03T13:08:54Z | |
dc.date.created | 2014-12-03T13:08:54Z | |
dc.date.issued | 2014-01-01 | |
dc.identifier | Process Biochemistry. Oxford: Elsevier Sci Ltd, v. 49, n. 1, p. 38-46, 2014. | |
dc.identifier | 1359-5113 | |
dc.identifier | http://hdl.handle.net/11449/111697 | |
dc.identifier | 10.1016/j.procbio.2013.09.024 | |
dc.identifier | WOS:000331156100006 | |
dc.identifier | 2115942621694174 | |
dc.identifier | 0000-0003-0195-3885 | |
dc.description.abstract | Biomolecules labeled with superparamagnetic nanoparticles can be selectively removed from complex reaction mixtures using an external magnetic field. Amino-functionalized superparamagnetic iron oxide nanoparticles (amino-SPION) were co-aggregated with penicillin G acylase and then cross-linked, generating magnetic cross-linked enzymes aggregates (M-CLEAs) that were quickly and efficiently recovered from the reaction medium by applying an external magnetic field. M-CLEAs and cross-linked enzymes aggregates (CLEAs) prepared under the same reaction conditions were characterized and compared. The best recovered activities were obtained for M-CLEAs prepared using polyethylene glycol 600 as precipitant and the most stable M-CLEA were obtained using tert-butanol. Successive penicillin G hydrolysis reactions were carried out using the same M-CLEA in a 50 mL reactor (3 reaction cycles), after the reactions the derivate was magnetically recovered without loss of activity demonstrating a total magnetic recovery. Line-scan energy dispersive X-ray spectroscopy showed that the amino-SPIONs were homogeneously dispersed within the structure of the M-CLEA. (C) 2013 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Process Biochemistry | |
dc.relation | 2.616 | |
dc.relation | 0,761 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Cross-linked enzymes aggregates | |
dc.subject | Superparamagnetic nanoparticles | |
dc.subject | Supramolecular complex | |
dc.subject | Magnetic recovery of enzymes | |
dc.subject | Penicillin hydrolysis | |
dc.title | Easily handling penicillin G acylase magnetic cross-linked enzymes aggregates: Catalytic and morphological studies | |
dc.type | Artículos de revistas | |