dc.contributorUniversity of Roma Tre
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorThe Tokyo University
dc.date.accessioned2014-05-27T11:30:15Z
dc.date.available2014-05-27T11:30:15Z
dc.date.created2014-05-27T11:30:15Z
dc.date.issued2013-08-27
dc.identifierSynthetic Biology, p. 261-276.
dc.identifierhttp://hdl.handle.net/11449/76335
dc.identifier10.1016/B978-0-12-394430-6.00014-5
dc.identifier2-s2.0-84882684210
dc.description.abstractThe construction of synthetic cells is one of the major goals of bioengineering. The most successful approach consists in the encapsulation of biochemical materials (DNA, RNA, enzymes, etc.) inside lipid vesicles (liposomes), mimicking a cell structure. In this contribution, that also aims at introducing the reader to 'chemical synthetic biology,' we describe the current state of the art of 'semi-synthetic minimal cells' (SSMCs), namely, cell-like structures containing the minimal number of biological compounds that are required to reconstruct a function of interest. We will first describe how the concept of the minimal cell was originated and its relation with the theory of autopoiesis, then we review the most advanced results focused on genetic/metabolic networks inside liposomes. Next, we emphasize that relevance of physical aspects (too often neglected) that impact on the solute entrapment process, and finally we discuss new technological trends in SSMC research that will probably allow their future use in biotechnology. © 2013 Copyright © 2013 Elsevier Inc. All rights reserved.
dc.languageeng
dc.relationSynthetic Biology
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAutopoiesis
dc.subjectCell-free
dc.subjectLiposomes
dc.subjectMinimal cell
dc.subjectOrigin of life
dc.subjectProtein synthesis
dc.subjectSelf-organization
dc.subjectSelf-reproduction
dc.subjectSynthetic biology
dc.titleSemi-synthetic minimal cells: Biochemical, physical, and technological aspects
dc.typeCapítulos de libros


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