dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:29:53Z
dc.date.available2014-05-27T11:29:53Z
dc.date.created2014-05-27T11:29:53Z
dc.date.issued2013-07-01
dc.identifierActa Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013.
dc.identifier0102-8650
dc.identifier1678-2674
dc.identifierhttp://hdl.handle.net/11449/75842
dc.identifier10.1590/S0102-86502013000700004
dc.identifierS0102-86502013000700004
dc.identifierWOS:000322407000004
dc.identifier2-s2.0-84880393431
dc.identifier2-s2.0-84880393431.pdf
dc.identifier2443296326760741
dc.identifier8727897080522289
dc.identifier9989857854355692
dc.identifier0000-0001-8411-5822
dc.description.abstractPurpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days.
dc.languageeng
dc.relationActa Cirúrgica Brasileira
dc.relation0.933
dc.relation0,395
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectHyperoxaluria
dc.subjectLithiasis
dc.subjectNephrocalcinosis
dc.subjectRats
dc.subjectcalcium
dc.subjectcalcium oxalate
dc.subjectcitric acid
dc.subjectcreatinine
dc.subjectoxalic acid
dc.subjecturic acid
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcontrolled study
dc.subjecthistology
dc.subjecthyperoxaluria
dc.subjectkidney calcification
dc.subjectkidney parenchyma
dc.subjectmale
dc.subjectmorphometrics
dc.subjectnonhuman
dc.subjectrat
dc.subjecturine pH
dc.subjecturine volume
dc.titleNephrocalcinosis induced by hyperoxaluria in rats
dc.typeArtículos de revistas


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