dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorICMBio
dc.date.accessioned2014-05-27T11:29:02Z
dc.date.available2014-05-27T11:29:02Z
dc.date.created2014-05-27T11:29:02Z
dc.date.issued2013-05-01
dc.identifierAquaculture Research, v. 44, n. 6, p. 876-884, 2013.
dc.identifier1355-557X
dc.identifier1365-2109
dc.identifierhttp://hdl.handle.net/11449/75259
dc.identifier10.1111/j.1365-2109.2012.03092.x
dc.identifierWOS:000318159400004
dc.identifier2-s2.0-84876811749
dc.identifier3815338174165832
dc.description.abstractHerein, we have developed molecular markers for nuclear genes to use in multiplex-PCR and PCR-RFLP, with the goal of characterising hybrid lines derived from crosses between pintado Pseudoplatystoma corruscans and cachara P. reticulatum. These markers, together with others described previously, were used to perform molecular identification analyses as genetic subsidies for Brazilian aquaculture. These analyses were performed due to the problems of high mortality in the offspring reported by the aquaculturist. From a total of 16 broodstock samples, 13 were genetically identified as hybrids; surprisingly, nine of these hybrids were found to be post-F1 lineages. These data show that the fertility of these animals can seriously affect the cultivated stocks, thus causing financial damage in this aquaculture system. The establishment of PCR-RFLP and multiplex-PCR as molecular techniques allows for both the correct management of these animals and the routine monitoring of production and trade of fish hybrids in aquaculture. Consequently, such tools will enable a sustainable development in the aquaculture industry. © 2012 Blackwell Publishing Ltd.
dc.languageeng
dc.relationAquaculture Research
dc.relation1.475
dc.relation0,586
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectGenetic identification
dc.subjectHybridization
dc.subjectPseudoplatystoma
dc.subjectSustainable development
dc.subjectaquaculture industry
dc.subjectaquaculture production
dc.subjectbiomarker
dc.subjectbrood stock
dc.subjecteconomic impact
dc.subjectgene expression
dc.subjecthyaloclastite
dc.subjecthybrid
dc.subjecthybridization
dc.subjectmolecular analysis
dc.subjectmortality
dc.subjectpolymerase chain reaction
dc.subjectsustainable development
dc.subjectBrazil
dc.subjectAnimalia
dc.subjectPseudoplatystoma corruscans
dc.titleDetection of post-F1 fish hybrids in broodstock using molecular markers: Approaches for genetic management in aquaculture
dc.typeArtículos de revistas


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