Validation of an alternative analytical method for the quantification of antioxidant activity in plant extracts

dc.contributorLaboratório de Cosmetologia-LaCos
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:55Z
dc.date.available2014-05-27T11:28:55Z
dc.date.created2014-05-27T11:28:55Z
dc.date.issued2013-04-15
dc.identifierLatin American Journal of Pharmacy, v. 32, n. 1, p. 90-95, 2013.
dc.identifier0326-2383
dc.identifierhttp://hdl.handle.net/11449/75120
dc.identifierWOS:000331245800013
dc.identifier2-s2.0-84876010393
dc.identifier3316011688829943
dc.identifier9734333607975413
dc.identifier4842462513285606
dc.identifier0000-0003-4141-0455
dc.description.abstractSUMMARY. A new method was developed to evaluate the total antioxidant activity in plant extracts, which was based on radical scavenging using 2,2'-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid. The proposed method was linear in the range of 0.86 to 26.0 μg/mL, displaying a correlation coefficient of 0.999. Moreover, the accuracy and precision analysis showed agreement with ANVISA guidelines (96.66 to 98.46% and < 5.0%, respectively). The method demonstrated sensitivity, robustness and efficiency in detecting low concentrations of plant extracts (detection and quantification limit of 0.86 and 2.87 μg/mL, respectively) that may be present in pharmaceuticals and cosmetics preparations, again in agreement with ANVISA. In addition, the assay is inexpensive and easy to perform.
dc.languageeng
dc.relationLatin American Journal of Pharmacy
dc.relation0.401
dc.relation0,152
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAntioxidant activity
dc.subjectPlant extracts
dc.subjectSpectrophotometry UV-Vis
dc.subject2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
dc.subjectantioxidant
dc.subjectAscophyllum nodosum extract
dc.subjectplant extract
dc.subjectunclassified drug
dc.subjectaccuracy
dc.subjectantioxidant activity
dc.subjectAscophyllum
dc.subjectascophyllum nodosum
dc.subjectdrug determination
dc.subjectdrug screening
dc.subjectdrug stability
dc.subjectinstrument validation
dc.subjectlimit of detection
dc.subjectlimit of quantitation
dc.subjectlinear system
dc.subjectphytochemistry
dc.subjectpractice guideline
dc.subjectprocess development
dc.subjectquantitative analysis
dc.subjectreproducibility
dc.subjectsensitivity analysis
dc.titleValidation of an alternative analytical method for the quantification of antioxidant activity in plant extracts
dc.typeArtículos de revistas


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