dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | University of Porto | |
dc.contributor | Universidade Federal de São Carlos (UFSCar) | |
dc.date.accessioned | 2014-05-27T11:27:17Z | |
dc.date.available | 2014-05-27T11:27:17Z | |
dc.date.created | 2014-05-27T11:27:17Z | |
dc.date.issued | 2012-12-01 | |
dc.identifier | Analytical Letters, v. 46, n. 2, p. 258-265, 2012. | |
dc.identifier | 0003-2719 | |
dc.identifier | 1532-236X | |
dc.identifier | http://hdl.handle.net/11449/73799 | |
dc.identifier | 10.1080/00032719.2012.713065 | |
dc.identifier | WOS:000312984400004 | |
dc.identifier | 2-s2.0-84871859504 | |
dc.identifier | 0477045906733254 | |
dc.identifier | 0000-0003-2827-0208 | |
dc.description.abstract | Electrogravimetric analysis was performed on the consumption of the neurotransmitter Acetylcholine (ACh) by Acetylcholinesterase (AChE) in situ and in real time. Michaelis-Menten assumption was achieved by using an enzyme micro-reactor in which the total enzyme was anchored in a quartz crystal microbalance chip (QCM-chip) with a strategically engineered self-assembled monolayer (SAM) of alkanethiols, which can prevent diffusion-controlled or spatially restricted kinetics. The real-time frequency changes indicated the rate of the products formation from enzymatic reaction. The QCM-chip was tested showing that it could demonstrate AChE inhibition by physostigmine. © 2013 Copyright Taylor and Francis Group, LLC. | |
dc.language | eng | |
dc.relation | Analytical Letters | |
dc.relation | 1.206 | |
dc.relation | 0,344 | |
dc.rights | Acesso restrito | |
dc.source | Scopus | |
dc.subject | Acetyl cholinesterase | |
dc.subject | Acetylcholine | |
dc.subject | Neurotransmitter | |
dc.subject | Physostigmine | |
dc.subject | Quartz-crystal microbalance | |
dc.title | Electrogravimetric Analysis by Quartz-Crystal Microbalance on the Consumption of the Neurotransmitter Acetylcholine by Acetylcholinesterase | |
dc.type | Artículos de revistas | |