Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:59Z
dc.date.available2014-05-27T11:25:59Z
dc.date.created2014-05-27T11:25:59Z
dc.date.issued2011-09-01
dc.identifierBrazilian Journal of Infectious Diseases, v. 15, n. 5, p. 478-481, 2011.
dc.identifier1413-8670
dc.identifier1678-4391
dc.identifierhttp://hdl.handle.net/11449/72656
dc.identifierS1413-86702011000500011
dc.identifier2-s2.0-82855182014
dc.identifier2-s2.0-82855182014.pdf
dc.description.abstractPhenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among Brazilian clinical isolates. © 2011 Elsevier Editora Ltda.
dc.languageeng
dc.relationBrazilian Journal of Infectious Diseases
dc.relation2.083
dc.relation0,817
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectCarbapenems
dc.subjectDrug resistance, bacterial
dc.subjectMetalloproteins
dc.subjectPolymerase chain reaction
dc.subjectPseudomonas aeruginosa
dc.subjectceftazidime
dc.subjectimipenem
dc.subjectimipenemase metallo beta lactamase
dc.subjectmercaptopropionic acid
dc.subjectmeropenem
dc.subjectmetallo beta lactamase
dc.subjectpolymyxin B
dc.subjectpropionic acid derivative
dc.subjectSao Paulo metallo beta lactamase
dc.subjectunclassified drug
dc.subjectantibiotic sensitivity
dc.subjectbacterial gene
dc.subjectbacterial transmission
dc.subjectbacterium isolate
dc.subjectbla IMP gene
dc.subjectbla SPM gene
dc.subjectblood culture
dc.subjectbody fluid
dc.subjectBrazil
dc.subjectcontrolled study
dc.subjectenzyme analysis
dc.subjectgenotype
dc.subjectminimum inhibitory concentration
dc.subjectmultidrug resistance
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpolymerase chain reaction
dc.subjectsensitivity and specificity
dc.subjecttertiary health care
dc.subjecttest strip
dc.subjectuniversity hospital
dc.subjecturine culture
dc.subjectAnti-Bacterial Agents
dc.subjectbeta-Lactamases
dc.subjectCeftazidime
dc.subjectCross Infection
dc.subjectGenes, Bacterial
dc.subjectGenotype
dc.subjectHospitals, Public
dc.subjectHumans
dc.subjectImipenem
dc.subjectMicrobial Sensitivity Tests
dc.subjectPhenotype
dc.subjectPolymerase Chain Reaction
dc.subjectThienamycins
dc.titleDetection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
dc.typeArtículos de revistas


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