Taenia solium and Taenia saginata: Identification of sequence characterized amplified region (SCAR) markers

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:34Z
dc.date.available2014-05-27T11:22:34Z
dc.date.created2014-05-27T11:22:34Z
dc.date.issued2007-09-01
dc.identifierExperimental Parasitology, v. 117, n. 1, p. 9-12, 2007.
dc.identifier0014-4894
dc.identifier1090-2449
dc.identifierhttp://hdl.handle.net/11449/69832
dc.identifier10.1016/j.exppara.2007.03.005
dc.identifierWOS:000249201800002
dc.identifier2-s2.0-34547092463
dc.identifier9991374083045897
dc.identifier1892359871207408
dc.description.abstractCysticercosis is one of the most important zoonosis, not only because of the effects on animal health and its economic consequences, but also due to the serious danger it poses to humans. The two main parasites involved in the taeniasis-cysticercosis complex in Brazil are Taenia saginata and Taenia solium. Differentiating between these two parasites is important both for disease control and for epidemiological studies. The purpose of this work was to identify genetic markers that could be used to differentiate these parasites. Out of 120 oligonucleotide decamers tested in random amplified polymorphic DNA (RAPD) assays, 107 were shown to discriminate between the two species of Taenia. Twenty-one DNA fragments that were specific for each species of Taenia were chosen for DNA cloning and sequencing. Seven RAPD markers were converted into sequence characterized amplified region (SCAR) markers with two specific for T. saginata and five specific for T. solium as shown by agarose gel electrophoresis. These markers were developed as potential tools to differentiate T. solium from T. saginata in epidemiological studies. © 2007 Elsevier Inc. All rights reserved.
dc.languageeng
dc.relationExperimental Parasitology
dc.relation1.821
dc.relation0,635
dc.relation0,635
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectCestode
dc.subjectdeoxyribonucleic acid
dc.subjectDNA
dc.subjectPCR
dc.subjectpolymerase chain reaction
dc.subjectrandom amplified polymorphic DNA
dc.subjectRAPD
dc.subjectSCAR
dc.subjectsequence characterized amplified region
dc.subjectTaenia saginata
dc.subjectTaenia solium
dc.subjectTaeniasis-cysticercosis complex
dc.subjectagarose
dc.subjectDNA fragment
dc.subjectoligonucleotide
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectgel electrophoresis
dc.subjectgenetic marker
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectparasite identification
dc.subjectpriority journal
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectCattle
dc.subjectDiagnosis, Differential
dc.subjectDNA, Helminth
dc.subjectGenetic Markers
dc.subjectMolecular Sequence Data
dc.subjectRandom Amplified Polymorphic DNA Technique
dc.subjectSpecies Specificity
dc.subjectSwine
dc.subjectTaeniasis
dc.subjectAnimalia
dc.subjectCestoda
dc.subjectTaenia
dc.titleTaenia solium and Taenia saginata: Identification of sequence characterized amplified region (SCAR) markers
dc.typeArtículos de revistas


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