Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:27Z
dc.date.available2014-05-27T11:22:27Z
dc.date.created2014-05-27T11:22:27Z
dc.date.issued2007-05-01
dc.identifierRevista da Sociedade Brasileira de Medicina Tropical, v. 40, n. 3, p. 277-281, 2007.
dc.identifier0037-8682
dc.identifierhttp://hdl.handle.net/11449/69639
dc.identifier10.1590/S0037-86822007000300005
dc.identifierS0037-86822007000300005
dc.identifierWOS:000248032900005
dc.identifier2-s2.0-34547161328
dc.identifier2-s2.0-34547161328.pdf
dc.description.abstractHTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.
dc.languagepor
dc.relationRevista da Sociedade Brasileira de Medicina Tropical
dc.relation1.358
dc.relation0,658
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectgp21
dc.subjectHeterologous expression
dc.subjectHTLV-1
dc.subjectRecombinant proteins
dc.subjectmembrane protein
dc.subjectmonoclonal antibody
dc.subjectvirus antibody
dc.subjectvirus glycoprotein
dc.subjectantigen antibody reaction
dc.subjectbiotechnology
dc.subjectdiagnostic kit
dc.subjectEscherichia coli
dc.subjectHuman T cell leukemia virus 1
dc.subjectmolecular cloning
dc.subjectprokaryote
dc.subjectprotein expression
dc.subjectvirus diagnosis
dc.subjectAntibodies, Monoclonal
dc.subjectCloning, Molecular
dc.subjectenv Gene Products, Human Immunodeficiency Virus
dc.subjectGene Expression
dc.subjectGene Products, env
dc.subjectGenetic Vectors
dc.subjectHTLV-I Antibodies
dc.subjectHuman T-lymphotropic virus 1
dc.subjectHumans
dc.subjectImmunoblotting
dc.subjectPolymerase Chain Reaction
dc.subjectProkaryota
dc.titleClonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto
dc.typeArtículos de revistas


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