Acute and short-term insulin-induced molecular adaptations of GLUT2 gene expression in the renal cortex of diabetic rats

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversity Foundation of Cardiology
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:22Z
dc.date.available2014-05-27T11:21:22Z
dc.date.created2014-05-27T11:21:22Z
dc.date.issued2005-06-15
dc.identifierMolecular and Cellular Endocrinology, v. 237, n. 1-2, p. 49-57, 2005.
dc.identifier0303-7207
dc.identifierhttp://hdl.handle.net/11449/68285
dc.identifier10.1016/j.mce.2005.03.005
dc.identifier2-s2.0-19544379373
dc.identifier0411008599070871
dc.identifier0000-0003-2145-6640
dc.description.abstractIncreased GLUT2 gene expression in the renal proximal tubule of diabetic rats is an adaptive condition, which may be important in the diabetic nephropathy development. We investigated the effects of insulin treatment upon the renal GLUT2 overexpression of diabetic rats. Acute treatment, surprisingly, induced a rapid further increase in GLUT2 mRNA content. Twelve hours after insulin injection, GLUT2 mRNA was twice the value of saline-injected rats (P < 0.001), when GLUT2 protein remained unchanged. In response to short-term treatment, both GLUT2 mRNA and protein were increased in 1-day treated rats (P < 0.05 versus saline-injected), decreasing after that, and reaching, within 6 days, values close to those of non-diabetic rats. Concluding, insulin treatment induced: initially, an additional upregulation of GLUT2 gene expression, involving posttranscriptional modulation; thereafter, downregulation of GLUT2 expression, which returns to non-diabetic levels. The former may be related to increased insulin concentration, the latter may be due to glycemic control. © 2005 Elsevier B.V. All rights reserved.
dc.languageeng
dc.relationMolecular and Cellular Endocrinology
dc.relation3.563
dc.relation1,629
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAlloxan
dc.subjectDiabetes
dc.subjectGlucose transporters
dc.subjectGLUT2 mRNA
dc.subjectGLUT2 protein (rat kidney)
dc.subjectInsulin treatment
dc.subjectNephropathy
dc.subjectglucose transporter 2
dc.subjectinsulin
dc.subjectmessenger RNA
dc.subjectsodium chloride
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdiabetes mellitus
dc.subjectdrug effect
dc.subjectgene expression regulation
dc.subjectgene overexpression
dc.subjectgenetic transcription
dc.subjectglucose blood level
dc.subjectglucosuria
dc.subjectinsulin treatment
dc.subjectkidney cortex
dc.subjectmale
dc.subjectnonhuman
dc.subjectNorthern blotting
dc.subjectpriority journal
dc.subjectrat
dc.subjectupregulation
dc.subjectWestern blotting
dc.subjectAdaptation, Physiological
dc.subjectAnimals
dc.subjectBlood Glucose
dc.subjectDiabetes Mellitus, Experimental
dc.subjectGene Expression
dc.subjectGlucose Transporter Type 2
dc.subjectHypoglycemic Agents
dc.subjectInsulin
dc.subjectKidney Cortex
dc.subjectMale
dc.subjectMonosaccharide Transport Proteins
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectTime Factors
dc.titleAcute and short-term insulin-induced molecular adaptations of GLUT2 gene expression in the renal cortex of diabetic rats
dc.typeArtículos de revistas


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