dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.date.accessioned | 2014-05-27T11:21:07Z | |
dc.date.available | 2014-05-27T11:21:07Z | |
dc.date.created | 2014-05-27T11:21:07Z | |
dc.date.issued | 2004-08-01 | |
dc.identifier | Archives of Dermatological Research, v. 296, n. 3, p. 112-119, 2004. | |
dc.identifier | 0340-3696 | |
dc.identifier | http://hdl.handle.net/11449/67806 | |
dc.identifier | 10.1007/s00403-004-0488-x | |
dc.identifier | 2-s2.0-4344634280 | |
dc.identifier | 8275401688702343 | |
dc.description.abstract | The biological effects of catecholamines in mammalian pigment cells are poorly understood. Our previous results showed the presence of α1-adrenoceptors in SK-Mel 23 human melanoma cells. The aims of this work were to (1) characterize catecholamine effects on proliferation, tyrosinase activity and expression, (2) identify the α1- adrenoceptor subtypes, and (3) verify whether chronic norepinephrine (NE) treatment modified the types and/or pharmacological characteristics of adrenoceptors present in SK-Mel 23 human melanoma cells. Cells treated with the aradrenergic agonist, phenylephrine (PHE, 10-5 or 10-4 M), for 24-72 h, exhibited decreased cell proliferation and enhanced tyrosinase activity, but unaltered tyrosinase expression as compared with the control. The proliferation and tyrosinase activity responses were inhibited by the α1-adrenergic antagonist prazosin, suggesting they were evoked by α1-adrenoceptors. The presence of actinomycin D, a transcription inhibitor, did not diminish PHE-induced effects. RT-PCR assays, followed by cloning and sequencing, demonstrated the presence of α1A- and α1B-adrenoceptor subtypes. NE-treated cells (24 or 72 h) were used in competition assays, and showed no significant change in the competition curves of α1-adrenoceptors as compared with control curves. Other adrenoceptor subtypes were not identified in these cells, and NE pretreatment did not induce their expression. In conclusion, the activation of SK-Mel 23 human melanoma α1- radrenoceptors elicit biological effects, such as proliferation decrease and tyrosinase activity increase. Desensitization or expression of other adrenoceptor subtypes after chronic NE treatment were not observed. | |
dc.language | eng | |
dc.relation | Archives of Dermatological Research | |
dc.relation | 2.148 | |
dc.relation | 1,006 | |
dc.rights | Acesso restrito | |
dc.source | Scopus | |
dc.subject | α1-Adrenoceptors | |
dc.subject | Catecholamines | |
dc.subject | Cell proliferation | |
dc.subject | SK-Mel 23 melanoma cells | |
dc.subject | Tyrosinase activity | |
dc.subject | alpha 1 adrenergic receptor | |
dc.subject | alpha 1 adrenergic receptor blocking agent | |
dc.subject | alpha 1 adrenergic receptor stimulating agent | |
dc.subject | ascorbic acid | |
dc.subject | benoxathian | |
dc.subject | catecholamine | |
dc.subject | dactinomycin | |
dc.subject | monophenol monooxygenase | |
dc.subject | noradrenalin | |
dc.subject | phenylephrine | |
dc.subject | prazosin | |
dc.subject | adrenergic system | |
dc.subject | catecholamine metabolism | |
dc.subject | cell proliferation | |
dc.subject | cell strain SK Mel 23 | |
dc.subject | controlled study | |
dc.subject | enzyme activity | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | melanoma cell | |
dc.subject | pigment cell | |
dc.subject | priority journal | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | Western blotting | |
dc.subject | Adrenergic alpha-Agonists | |
dc.subject | Binding, Competitive | |
dc.subject | Cell Division | |
dc.subject | Cell Line, Tumor | |
dc.subject | Gene Expression | |
dc.subject | Humans | |
dc.subject | Melanoma | |
dc.subject | Monophenol Monooxygenase | |
dc.subject | Norepinephrine | |
dc.subject | Phenylephrine | |
dc.subject | Receptors, Adrenergic, alpha-1 | |
dc.subject | Skin Neoplasms | |
dc.title | Catecholamine effects on human melanoma cells evoked by α1-adrenoceptors | |
dc.type | Artículos de revistas | |