Two Digestive Trypsins Occur in Three Species of Neotropical Anophelines

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversity of California
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:56Z
dc.date.available2014-05-27T11:20:56Z
dc.date.created2014-05-27T11:20:56Z
dc.date.issued2003-11-01
dc.identifierJournal of Medical Entomology, v. 40, n. 6, p. 991-995, 2003.
dc.identifier0022-2585
dc.identifierhttp://hdl.handle.net/11449/67468
dc.identifier2-s2.0-1642529555
dc.description.abstractTrypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Amino-terminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.
dc.languageeng
dc.relationJournal of Medical Entomology
dc.relation1.968
dc.relation0,962
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAnopheles
dc.subjectMalaria vector
dc.subjectTrypsin
dc.subjecttrypsin
dc.subjectamino acid sequence
dc.subjectanimal
dc.subjectblood
dc.subjectBrazil
dc.subjectchemistry
dc.subjectclassification
dc.subjectcomparative study
dc.subjectdigestion
dc.subjectenzymology
dc.subjectfeeding behavior
dc.subjectgenetics
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectnucleotide sequence
dc.subjectparasitology
dc.subjectphysiology
dc.subjectsensitivity and specificity
dc.subjectsequence alignment
dc.subjectsequence homology
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBlood
dc.subjectConserved Sequence
dc.subjectDigestion
dc.subjectFeeding Behavior
dc.subjectHumans
dc.subjectKinetics
dc.subjectMolecular Sequence Data
dc.subjectSensitivity and Specificity
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectAlbitarsis
dc.subjectAnimalia
dc.subjectAnopheles albitarsis
dc.subjectAnopheles aquasalis
dc.subjectAnopheles darlingi
dc.subjectHexapoda
dc.titleTwo Digestive Trypsins Occur in Three Species of Neotropical Anophelines
dc.typeArtículos de revistas


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