Araçatuba virus: A vaccinialike virus associated with infection in humans and cattle

dc.contributorUniversidade Federal de Minas Gerais (UFMG)
dc.contributorNational Institutes of Health
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorInstituto Biológico
dc.date.accessioned2014-05-27T11:20:36Z
dc.date.available2014-05-27T11:20:36Z
dc.date.created2014-05-27T11:20:36Z
dc.date.issued2003-02-01
dc.identifierEmerging Infectious Diseases, v. 9, n. 2, p. 155-160, 2003.
dc.identifier1080-6040
dc.identifierhttp://hdl.handle.net/11449/67194
dc.identifier2-s2.0-0344211874
dc.identifier2232625970712015
dc.identifier2976129874554698
dc.description.abstractWe describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
dc.languageeng
dc.relationEmerging Infectious Diseases
dc.relation7.422
dc.relation3,278
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectanimal cell
dc.subjectAracatuba virus
dc.subjectcattle disease
dc.subjectcontrolled study
dc.subjectcowpox
dc.subjectDNA sequence
dc.subjectDNA virus
dc.subjectgene amplification
dc.subjectgene cluster
dc.subjectgene deletion
dc.subjectgene sequence
dc.subjectmolecular biology
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectOrthopoxvirus
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectsequence analysis
dc.subjectsequence homology
dc.subjectVaccinia virus
dc.subjectVero cell
dc.subjectvirogenesis
dc.subjectvirus gene
dc.subjectvirus identification
dc.subjectvirus infection
dc.subjectvirus isolation
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectCommunicable Diseases, Emerging
dc.subjectGenes, Viral
dc.subjectHumans
dc.subjectMicroscopy, Electron
dc.subjectMolecular Sequence Data
dc.subjectPhylogeny
dc.subjectSequence Alignment
dc.subjectVaccinia
dc.subjectViral Proteins
dc.subjectViral Structural Proteins
dc.subjectViremia
dc.titleAraçatuba virus: A vaccinialike virus associated with infection in humans and cattle
dc.typeArtículos de revistas


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