SISTEMAS QUIMILUMINESCENTES COM PEROXIDASE (EC:1.11.1.7) E SUAS APLICACOES EM ANALISES CLINICAS

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:17:59Z
dc.date.available2014-05-27T11:17:59Z
dc.date.created2014-05-27T11:17:59Z
dc.date.issued1995-01-01
dc.identifierRevista de Ciencias Farmaceuticas, v. 16, p. 55-77.
dc.identifier0101-3793
dc.identifierhttp://hdl.handle.net/11449/64574
dc.identifier2-s2.0-0028789494
dc.description.abstractThe enzyme horseradish peroxidase HRP (EC:1.11.1.7), has both acid and basic isoenzymes, catalyses a wide range of reactions (acting as an oxiredutase or an oxidase) and is thought capable of one- or two-electrons oxidations depending on the substrate employed. Today, the methodology for these assay can be chemiluminescent reactions and enhanced chemiluminescent. The enhanced chemiluminescent assay with system HRP, luminol, peroxide and an enhancer has provided the basis for a convenient and sensitive assay for peroxidase and peroxidase conjugates, DNA probe and blotting assay. It is particularly more advantageous than the others, because is very rapid, more sensitive (attomoles), easy to do and technically simple, and is relatively specific for HRP (reduces the effect of the interference).
dc.languagepor
dc.relationRevista de Ciencias Farmaceuticas
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectchemiluminescence
dc.subjectenhanced chemiluminescence
dc.subjectenzymes
dc.subjectluminol
dc.subjectperoxidase
dc.subjecthorseradish peroxidase
dc.subjectperoxide
dc.subjectchemoluminescence
dc.subjectenzyme analysis
dc.subjectenzyme assay
dc.subjectreview
dc.titleSISTEMAS QUIMILUMINESCENTES COM PEROXIDASE (EC:1.11.1.7) E SUAS APLICACOES EM ANALISES CLINICAS
dc.typeOtros


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