Characterization of myeloid or lymphoid acute leukemia by a chemiluminescence assay: Comparison with immunocytochemistry using an antimyeloperoxidase antibody

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:17:30Z
dc.date.available2014-05-27T11:17:30Z
dc.date.created2014-05-27T11:17:30Z
dc.date.issued1993-01-01
dc.identifierActa Haematologica, v. 90, n. 1, p. 19-24, 1993.
dc.identifier0001-5792
dc.identifierhttp://hdl.handle.net/11449/64338
dc.identifier10.1159/000204367
dc.identifierWOS:A1993MH30300005
dc.identifier2-s2.0-0027484569
dc.identifier4419635633356792
dc.description.abstractA simple and sensitive chemiluminescence assay for the demonstration of the activity of intracellular myeloperoxidase (MPO) is described, which is useful for the distinction between myeloid and lymphoid commitment in blasts from acute leukemia patients. When the cut-off point was settled at 13 mV of chemiluminescence all cases of acute myeloid leukemia (AML) were distinguished from those of acute lymphoid leukemia. In addition, this technique was able to demonstrate MPO activity in AML poorly differentiated (FAB-M0) which usually does not stain for MPO in classical cytochemistry preparations and could be negative also by immunocytochemistry with anti-MPO monoclonal antibody. Therefore the method here described presented a higher sensitivity than the immunocytochemistry procedure with anti-MPO.
dc.languageeng
dc.relationActa Haematologica
dc.relation1.307
dc.relation0,575
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAcute leukemia
dc.subjectAntimyeloperoxidase
dc.subjectChemiluminescence
dc.subjectMyeloperoxidase
dc.subjectmyeloperoxidase
dc.subjectacute granulocytic leukemia
dc.subjectacute lymphocytic leukemia
dc.subjectadolescent
dc.subjectadult
dc.subjectaged
dc.subjectbone marrow biopsy
dc.subjectchemoluminescence
dc.subjectchild
dc.subjectcontrolled study
dc.subjectdiagnostic accuracy
dc.subjectenzyme activity
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunocytochemistry
dc.subjectinfant
dc.subjectintermethod comparison
dc.subjectmajor clinical study
dc.subjectpriority journal
dc.subjectAcute Disease
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectAntibodies, Monoclonal
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectComparative Study
dc.subjectDiagnosis, Differential
dc.subjectHuman
dc.subjectImmunohistochemistry
dc.subjectImmunophenotyping
dc.subjectInfant
dc.subjectLeukemia, Lymphocytic, Acute
dc.subjectLeukemia, Myeloid
dc.subjectMiddle Age
dc.subjectPeroxidase
dc.subjectSensitivity and Specificity
dc.subjectSupport, Non-U.S. Gov't
dc.titleCharacterization of myeloid or lymphoid acute leukemia by a chemiluminescence assay: Comparison with immunocytochemistry using an antimyeloperoxidase antibody
dc.typeArtículos de revistas


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