dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Sorocaba
dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:33:16Z
dc.date.available2014-05-20T15:33:16Z
dc.date.created2014-05-20T15:33:16Z
dc.date.issued2011-03-01
dc.identifierJournal of Polymers and The Environment. New York: Springer/plenum Publishers, v. 19, n. 1, p. 196-202, 2011.
dc.identifier1566-2543
dc.identifierhttp://hdl.handle.net/11449/41942
dc.identifier10.1007/s10924-010-0262-4
dc.identifierWOS:000289801700025
dc.description.abstractThis work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions containing three different concentrations of nanoparticles (5.4, 54 and 540 mu g/mL, polymer mass/volume of solution). The nanoparticles were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95 nm, a zeta potential of -20 mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations employed. The results provide new information concerning the toxic effects of particles produced using PLGA.
dc.languageeng
dc.publisherSpringer/plenum Publishers
dc.relationJournal of Polymers and the Environment
dc.relation1.971
dc.relation0,562
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPolymeric nanoparticles
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectEnvironmental toxicity
dc.titleEvaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticles
dc.typeArtículos de revistas


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