A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Munster
dc.date.accessioned2014-05-20T15:33:01Z
dc.date.available2014-05-20T15:33:01Z
dc.date.created2014-05-20T15:33:01Z
dc.date.issued2011-03-01
dc.identifierMitochondrion. Oxford: Elsevier B.V., v. 11, n. 2, p. 296-302, 2011.
dc.identifier1567-7249
dc.identifierhttp://hdl.handle.net/11449/41762
dc.identifier10.1016/j.mito.2010.12.010
dc.identifierWOS:000287112100008
dc.description.abstractSNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationMitochondrion
dc.relation3.226
dc.relation1,666
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAdmixture populations
dc.subjectBrazil
dc.subjectHaplogroup affiliation
dc.subjectMitochondrial DNA
dc.subjectSNaPshot reaction
dc.titleA single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups
dc.typeArtículos de revistas


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