Artículos de revistas
Inorganic-Organic Nanocomposite Assembly Using Collagen as a Template and Sodium Tripolyphosphate as a Biomimetic Analog of Matrix Phosphoprotein
Fecha
2011-08-01Registro en:
Crystal Growth & Design. Washington: Amer Chemical Soc, v. 11, n. 8, p. 3504-3511, 2011.
1528-7483
10.1021/cg200663v
WOS:000293356400025
1754020652874850
0000-0003-4830-0400
Autor
Sun Yat Sen Univ
First Hosp Wuhan
Fourth Mil Med Univ
Peking Univ
Universidade Estadual Paulista (Unesp)
Georgia Hlth Sci Univ
Institución
Resumen
Nanocomposites created with polycarboxylic acid alone as a stabilization agent for prenucleation clusters-derived amorphous calcium phosphate exhibit nonperiodic apatite deposition. In the present study, we report the use of inorganic polyphosphate as a biomimetic analog of matrix phosphoprotein for directing poly(acrylic acid)-stabilized amorphous nano-precursor phases to assemble into periodic apatite-collagen nanocomposites. The sorption and desorption characteristics of sodium tripolyphosphate to type I collagen were examined. Periodic nanocomposite assembly with collagen as a template was demonstrated with TEM and SEM using a Portland cement-based resin composite and a phosphate-containing simulated body fluid. Apatite was detected within the collagen at 24 h and became more distinct at 48 h, with prenucleation clusters attaching to the collagen fibril surface during the initial infiltration stage. Apatite-collagen nanocomposites at 72 h were heavily mineralized with periodically arranged intrafibrillar apatite platelets. Defect-containing nanocomposites caused by desorption of TPP from collagen fibrils were observed in regions lacking the inorganic phase.