dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorComis Nacl Energia Atom
dc.date.accessioned2014-05-20T15:30:12Z
dc.date.available2014-05-20T15:30:12Z
dc.date.created2014-05-20T15:30:12Z
dc.date.issued2005-08-08
dc.identifierAnalytica Chimica Acta. Amsterdam: Elsevier B.V., v. 546, n. 2, p. 244-250, 2005.
dc.identifier0003-2670
dc.identifierhttp://hdl.handle.net/11449/39631
dc.identifier10.1016/j.aca.2005.05.030
dc.identifierWOS:000230684100015
dc.description.abstractA study was undertaken to evaluate Saccharonzyces cerevisiae as a substrate for the biosorption of Cr(III) and Cr(VI) aiming to the selective determination of these species in aqueous solutions. The yeast cells were covalently immobilised on controlled pore glass (CPG), packed in a minicolumn and incorporated in an on-line flow injection system. The effect of chemical and physical variables affecting the biosorption process was tested in order to select the optimal analytical conditions for the Cr retention by S. cerevisiae. Cr(III) was retained by the immobilised cells and Cr(VI) were retained by CPG. The speciation was possible by selective and sequential elution of Cr(III) with 0.05 mol L-1 HCl and 2.0 mol L-1 HNO3 for Cr(VI). The influence of some concomitant ions up to 20 mg L-1 was also tested. Quantitative determinations of Cr were carried out by means of inductively coupled plasma optical emission spectrometry (ICP OES). Preconcentration factors of 12 were achieved for Cr(III) and 5 for Cr(VI) when 1.7 mL of sample were processed reaching detection limits of 0.45 for Cr(III) and 1.5 mu g L-1 for Cr(VI). The speciation of inorganic Cr in different kinds of natural waters was performed following the proposed method. Spiked water samples were also analysed and the recoveries were in all cases between 81 and 103%. (c) 2005 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationAnalytica Chimica Acta
dc.relation5.123
dc.relation1,512
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCr(III)
dc.subjectCr(VI)
dc.subjectSaccharomyces cerevisiae
dc.subjectimmobilisation
dc.subjectcontrolled pore glass
dc.subjectpreconcentration
dc.subjectspeciation analysis
dc.titleOn-line preconcentration and speciation analysis of Cr(III) and Cr(VI) using baker's yeast cells immobilised on controlled pore glass
dc.typeArtículos de revistas


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