dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Instituto Butantan | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.date.accessioned | 2014-05-20T15:25:37Z | |
dc.date.available | 2014-05-20T15:25:37Z | |
dc.date.created | 2014-05-20T15:25:37Z | |
dc.date.issued | 2003-08-01 | |
dc.identifier | Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 12, n. 4, p. 237-246, 2003. | |
dc.identifier | 0962-9351 | |
dc.identifier | http://hdl.handle.net/11449/35993 | |
dc.identifier | 10.1080/09629350310001599684 | |
dc.identifier | WOS:000185506700007 | |
dc.identifier | WOS000185506700007.pdf | |
dc.identifier | 5102737730539655 | |
dc.description.abstract | Background: Rat trachea display a differential topographical distribution of connective tissue mast cells (CTMC) and mucosal mast cells (MMC) that may imply regional differences in the release of allergic mediators such as tumor necrosis factor-alpha (TNF-alpha) and eicosanoids.Aim: To evaluate the role of CTMC and MMC for release of TNF-alpha and eicosanoids after allergenic challenge in distinct segments of rat trachea.Materials and methods: Proximal trachea ( PT) and distal trachea (DT) from ovalbumin (OVA)-sensitized rats, treated or not with compound 48/80 ( 48/80) or dexamethasone, were incubated in culture medium. After OVA challenge, aliquots were collected to study release of TNF-alpha and eicosanoids.Results: Release of TNF-alpha by PT upon OVA challenge peaked at 90 min and decayed at 6 and 24 h. Release from DT peaked at 30-90 min and decayed 6 and 24 h later. When CTMC were depleted with 48/80, OVA challenge exacerbated the TNF-alpha release by PT at all time intervals, while DT exacerbated TNF-alpha levels 6 and 24 h later only. Dexamethasone reduced TNF-alpha production after 90 min of OVA challenge in PT and at 3 and 6h in DT. OVA challenge increased prostaglandin D-2 in DT and leukotriene B-4 in both segments but did not modify prostaglandin E-2 and leukotriene C-4 release.Conclusion: OVA challenge induces TNF-alpha release from MMC, which is negatively regulated by CTMC. The profile of TNF-alpha and eicosanoids depends on the time after OVA challenge and of the tracheal segment considered. | |
dc.language | eng | |
dc.publisher | Carfax Publishing | |
dc.relation | Mediators of Inflammation | |
dc.relation | 3.549 | |
dc.relation | 1,370 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | TNF | |
dc.subject | eicosanoids | |
dc.subject | Mast cells | |
dc.subject | airways | |
dc.subject | asthma | |
dc.subject | rat | |
dc.title | Differential regulation of the release of tumor necrosis factor-alpha and of eicosanoids by mast cells in rat airways after antigen challenge | |
dc.type | Artículos de revistas | |