Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:56:04Z
dc.date.available2014-05-20T13:56:04Z
dc.date.created2014-05-20T13:56:04Z
dc.date.issued2005-05-01
dc.identifierBrazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.
dc.identifier1516-8913
dc.identifierhttp://hdl.handle.net/11449/20054
dc.identifier10.1590/S1516-89132005000300001
dc.identifierS1516-89132005000300001
dc.identifierWOS:000229613100001
dc.identifier2-s2.0-27944503315
dc.identifierWOS000229613100001.pdf
dc.identifier4110421764783871
dc.description.abstractRhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.
dc.languageeng
dc.publisherInst Tecnologia Parana
dc.relationBrazilian Archives of Biology and Technology
dc.relation0.676
dc.relation0,281
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectalkaline xylanase
dc.subjectRhizopus stolonifer
dc.subjectsolid-state fermentation
dc.titlePartial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
dc.typeArtículos de revistas


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